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BIOCHEMISTRY MODULE Enzymes Biochemistry 112 Notes OBJECTIVES After reading this lesson, you will be able to: zdefine enzymes zclassify enzymes zexplain co-enzymes zexplain the factors affecting enzyme activity zdescribe isoenzymes zexplain the Clinical significance of enzymes 8.2 DEFINITION AND CHARACTERISTICS OF ENZYMES • They are neither consumed nor permanently altered as a consequence of their participation in a reaction. The reaction converted hydrogen peroxide to water and oxygen and oxygen production was used as a measure of enzyme activity. Factors affecting enzyme activity Namrata Chhabra 30-Mar-18 Namrata Chhabra 1 2. Factors Affecting Enzymatic Activity – PPT (Temperature, Water, pH, [Substrate], [Enzyme]) Lecture Note on Factors Affecting Enzymatic Activity. Ø A small amount of enzyme is enough for a large amount of substrate. 3 INTRODUCTION TO ENZYMES Worthington Biochemical Corporation 800.445.9603 Enzymes and Life Processes The living cell is the site of tremendous biochemical activity called metabolism. (5). Factors affecting enzyme activity 1. The enzyme we studied was hydrogen peroxidase from a cow. 1 What are the factors affecting Enzyme Activity? PPT – Lecture 3: Factors affecting enzyme activity: [substrate] and inhibitors PowerPoint presentation | free to view - id: 1abb51-ZDc1Z. 1. 20.3 Factors Affecting Enzyme Activity The activity of an enzyme describes how fast an enzyme catalyzes the reaction and is strongly affected by reaction conditions, such as temperature. Ø Enzymes have extraordinary catalytic power. ... Factors Affecting Enzyme Activity. Make a dilution of the enzyme so that you have 3 strengths of enzyme: one at 200% enzyme strength (~4 mL solution), one at 100% enzyme strength (~4 mL), and one at 2 Recap 3 What are enzymes?
• Enzymes are biological catalysts of the chemical reactions. Factors affecting EnzymeCatalyzed Reactions 2. Factors affecting enzyme function Temperature Optimum T° greatest number of molecular collisions human enzymes = 35°- 40°C body temp = 37°C Heat: increase beyond optimum T° increased energy level of molecules disrupts bonds in enzyme & between enzyme & substrate H, ionic = weak bonds

Knowledge of basic enzyme kinetic theory is important in enzyme analysis in order both to understand the basic enzymatic mechanism and to select a method for enzyme analysis. Concentration of Enzyme. January 1993. Begin with the enzyme solution. What are Enzymes ? Question #1: What is the effect of enzyme concentration on enzyme activity? Biological catalysts made up of proteins; 4 Function of Enzymes.
Psychological Factors Affecting Physical Conditions. 2. ADVERTISEMENTS: This article throws light upon the six factors affecting the enzyme activity. Factors Affecting Enzyme Activity by John Eed (Biology 1151) Abstract: e studied the effect of temperature, enzyme concentration and pH on enzyme activity. Enzymes speed up the rate of chemical reactions in the body both breaking down (e.g. Set up 3 fresh cups of 1% H 2 O 2 that are 4 cm deep. starch into maltose) and building up reactions. Factors affecting Enzyme-Catalyzed Reactions 1- Effect of Substrate Concentration (Single-Substrate Reactions) 2- Effect of Inhibitors 3- Effect of pH 4- Effect of Temperature 5- Effect of Pressure 6- Effect of Water 3. The Adobe Flash plugin … Scribd … Factors affecting enzymes 1. The six factors are: (1) Concentration of Enzyme (2) Concentration of Substrate (3) Effect of Temperature (4) Effect of pH (5) Effect of Product Concentration and (6) Effect of Activators. Introduction to Enzymes.
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